Addgene

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pAS1-rab-3P::his-24::tagBFP

Plasmid

#124343

Purpose

Nuclear Localized Pan-Neuronal tagBFP

Depositor

Andrew Leifer

Insert

rab-3, tagBFP (rab-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBCN27-Pmyo-2::GFP::unc-54_3'UTR

Plasmid

#26347

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2010 Aug 22. ():.

Insert

Pmyo-2::GFP::unc-54_3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pDD285

Plasmid

#66826

Purpose

mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mKate2

Expression

Worm

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
Punc-4::NGL-1::spGFP1-10

Plasmid

#65827

Purpose

For trans-synaptic labeling

Depositor

Kang Shen, Cori Bargmann

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Use

Tags

spGFP1-10

Expression

Worm

Mutation

Promoter

Punc-4

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSH9

Plasmid

#90328

Purpose

Expresses the set-2.a cDNA isoform in the C. elegans intestine

Depositor

Anne Brunet

Article

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

Pges-1::set-2a

Use

Tags

Expression

Worm

Mutation

Promoter

ges-1

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ352

Plasmid

#30539

Depositor

Erik Jorgensen

Insert

ttTi4348 region

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

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pNH13 (pmyo-2::QuasAr::mOrange)

Plasmid

#130273

Purpose

Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr::mOrange

Use

Tags

mOrange

Expression

Worm

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Tags

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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Pvha6-AgDD

Plasmid

#80626

Purpose

create protein aggregates in C. elegans

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Use

Tags

MLALKLAGLDI

Expression

Worm

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Use

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Mutation

Promoter

myo-3 and unc-119

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY46

Plasmid

#191050

Purpose

glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-1 (glr-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY38

Plasmid

#191042

Purpose

unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-8 (unc-8 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY14

Plasmid

#191059

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5(orf) (acr-5 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY13

Plasmid

#191019

Purpose

Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

arrd-4 (ZK938.2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY20

Plasmid

#191026

Purpose

gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-18 (gcy-18 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY41

Plasmid

#191045

Purpose

dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dat-1 (dat-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY43

Plasmid

#191047

Purpose

eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

eat-4(prom6-1) (eat-4 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHygRR2

Plasmid

#200075

Purpose

hygR selection mScarlet 2X marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Tags

Expression

Worm

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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