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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSN444

Plasmid

#108331

Purpose

Expresses Sat2 for Nourseothricin resistance selection of C. elegans

Depositor

Shinsuke Niwa

Article

Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

Insert

Streptothricin acetyl transferase 2

Use

Tags

Expression

Worm

Mutation

Promoter

rps-27 promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrII_ttTi5605.sgRNA

Plasmid

#194058

Purpose

sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrII_ttTi5605 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrV_oxTi365.gRNA

Plasmid

#194052

Purpose

sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrV_oxTi365 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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BSP611

Plasmid

#122256

Purpose

expression vector for mtagRFP-T under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagRFP-T

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHygCG2r

Plasmid

#200074

Purpose

hygR selection mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Tags

Expression

Worm

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

Plasmid

#107133

Purpose

cGAL-N driver under the control of C. elegans hsp-16.41 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Use

Tags

Expression

Worm

Mutation

Promoter

C. elegans hsp-16.41 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHW581

Plasmid

#198818

Purpose

In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJF6

Plasmid

#139040

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

gfp (includes STOP codon, PATC introns)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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TC358

Plasmid

#164885

Purpose

TRE::GFP, inducible GFP expression when co-express with rtTA

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGC257

Plasmid

#19660

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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sgRNA with U6 promoter

Plasmid

#48962

Purpose

to drive the sgRNA expression under a U6 promoter

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pEG476

Plasmid

#40198

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S404A) (mex-5 Nematode)

Use

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

S404A

Promoter

pie-1

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pGC381

Plasmid

#19663

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*-CFP::let-858(3’)

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pSDH61

Plasmid

#139033

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (no STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJW2341

Plasmid

#186950

Purpose

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Depositor

Jordan Ward

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY405

Plasmid

#172694

Purpose

Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

rab-3 (rab-3 Nematode)

Use

Tags

Punc-25 and mcherry

Expression

Worm

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ9

Plasmid

#198786

Purpose

Drives specific expression in the AWC (ON) neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrt-28-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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