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Showing: 221 - 240 of 1700 results

  6. pSLGCV

    Plasmid
    #49862
    Purpose
    Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).
    Depositor
    Jonathan Pettitt, Anne Glover
    Article
    Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118
    Insert
    firefly luciferase luc+ fused to GFP (S65C)
    Use
    Tags
    GFP (S65C)
    Expression
    Worm
    Mutation
    Promoter
    sur-5
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49862-plasmid-map-sequence-id-74359

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  7. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

    Plasmid
    #182352
    Purpose
    Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182352-plasmid-map-sequence-id-361686

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  8. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid
    #47550
    Purpose
    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    ttTi5605 sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47550-plasmid-map-sequence-id-67865

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  11. pDD284

    Plasmid
    #66825
    Purpose
    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  13. pDD400

    Plasmid
    #137024
    Purpose
    SapTrap mNG-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137024-plasmid-map-sequence-id-274031

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Showing: 221 - 240 of 1700 results