Showing: 1 - 20 of 1631 results
-
Plasmid#85583PurposecGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)DepositorInsertPrab-3-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterPrab-3-GAL4-SK(DBD)-VP64AvailabilityAcademic Institutions and Nonprofits only -
pHW394 (15xUAS::GFP::let-858 3'UTR)
Plasmid#85584PurposecGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegansDepositorInsert15xUAS-delta pes-10-GFP
UseTagsExpressionWormMutationGFP(S65C) with three introns for expression in C.…Promoter15xUAS-delta pes-10-GFPAvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
UseTagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.PromoterAvailabilityAcademic Institutions and Nonprofits only -
BSP179
Plasmid#110915PurposeFor cloning expression cassettes by HR in yeast, Universal MosSCI compatibleDepositorTypeEmpty backboneUseSynthetic Biology; Yeast homologous recombination…TagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
BSP188
Plasmid#110917PurposeFor cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator clonedDepositorInsertunc54 Terminator
UseSynthetic Biology; Yeast homologous recombination…TagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)
Plasmid#107739Purposemutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein(C1A)
UseTagsExpressionWormMutationchange the first cysteine of gp41-1 N-intein to a…PromoterC. elegans myo-2 promoterAvailabilityAcademic Institutions and Nonprofits only -
pmec-4::Chrimson::SL2::mCherry::unc-54
Plasmid#107745PurposeExpress Chrimson and mCherry in mechanosensory neurons.DepositorInsertsChrimson
mCherry
UseTagsExpressionInsect and WormMutationPromotermec-4AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-GFP(dpiRNA)::CDK-1
Plasmid#107938Purposecdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertGFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
UseTagsExpressionWormMutationPromotercdk-1AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
UseTagsExpressionWormMutationPromoterpie-1AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
UseTagsExpressionWormMutationPromotereft-3AvailabilityAcademic Institutions and Nonprofits only -
pSN444
Plasmid#108331PurposeExpresses Sat2 for Nourseothricin resistance selection of C. elegansDepositorInsertStreptothricin acetyl transferase 2
UseTagsExpressionWormMutationPromoterrps-27 promoterAvailabilityAcademic Institutions and Nonprofits only -
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
Plasmid#108362PurposeExpression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegansDepositorInsertsUseTags2xFlag, MYC, T2A peptide, and TY1 linkerExpressionWormMutationPromoterA rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…AvailabilityAcademic Institutions and Nonprofits only -
Pmyo-3::tag:SL1(G20A)
Plasmid#81114PurposeExpression of exogenous SL1(G20A) in C. elegans muscleDepositorInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG20APromoterpromoter of myo-3AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
UseTagsExpressionWormMutationPromoterodr-3AvailabilityAcademic Institutions and Nonprofits only -
-
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1320
Plasmid#84829PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3AvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 1631 results