-
Plasmid#116010Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB401+Citrine
Plasmid#128432PurposeModified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.DepositorTypeEmpty backboneUseGateway destination vectorTagsCitrine, yellow fluorescence proteinExpressionPlantMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHWBF06exp
Plasmid#130831PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseTagsmCherry-FLAG-HAExpressionPlantMutationPromoterCaMV 35SAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH88
Plasmid#128201PurposesgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)DepositorInsertsgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:(MCS)-3xHA-S11
Plasmid#108185PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
Plasmid#172317PurposeCRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TF101-STTM-miR165/166
Plasmid#84816PurposeTurning off/down miR165/166 in SoybeanDepositorInsertSTTM-miR165/166
UseTagsExpressionPlantMutationPromoterunknownAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MIGS5.2 (FF576)
Plasmid#35254DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM829.2
Plasmid#84158Purposetarget miRNA829.2 for destruction in Arabidopsis thalianaDepositorInsertSTTM829.2
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN2
Plasmid#96977PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in plants.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN1
Plasmid#96976PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-GFP1–10
Plasmid#125664PurposeBinary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertER-GFP1–10
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB216
Plasmid#68607PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromoterAtuMASAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB200
Plasmid#68591PurposeMCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_1
Plasmid#91165PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [2_n-1] (GB1206)
Plasmid#75407PurposetRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)DepositorInserttRNA-gRNA position [2_n-1]
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB423
Plasmid#68688PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZA71
Plasmid#174785PurposeIn planta gene expressionDepositorInsertGG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker
UseTags3xFLAGExpressionPlantMutationWTPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA68
Plasmid#174781PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker
UseTagseGFPExpressionPlantMutationL17E/D481VPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA66
Plasmid#174779PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker
UseTagseGFPExpressionPlantMutationD481VPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA65
Plasmid#174778PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-eGFP, Kmr plant selection marker
UseTagseGFPExpressionPlantMutationWTPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA67
Plasmid#174780PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker
UseTagseGFPExpressionPlantMutationL17EPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTX208
Plasmid#89268PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02DepositorInsertOsYSA-gRNA01 and OsYSA-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterMaize Ubiquitin1 promoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)
Plasmid#160635PurposeTU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminatorDepositorInsertAcrIIA4
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2251
Plasmid#141305PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneUseTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB106-OEP7(1–50)
Plasmid#100602PurposeBinary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plantsDepositorInsertOEP7(1–50)-Citrine (OEP7 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEELA
Plasmid#79752Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xPro35SAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)
Plasmid#160641PurposeModule for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.DepositorInsertERLexABDGal4AD / PhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MIGS4.2 (FF575)
Plasmid#35252DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pG20_LUC2_Hyg
Plasmid#159707PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsluciferaseExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_GUS_Blp
Plasmid#159706PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsGUSExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
35S-frGFP-nosT
Plasmid#80128PurposeTransient expression vector for frGFP in plantsDepositorTypeEmpty backboneUseTagsfrGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEE386
Plasmid#149284PurposeT-DNA encoding TRV2 with gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE388
Plasmid#149286PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_mFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE385
Plasmid#149289PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA2_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd SUP
Plasmid#115489PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC50
Plasmid#104824PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.08g081600, Glyma.05g126600
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJG493
Plasmid#91197PurposeWDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)DepositorInsertgUbi1+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG20_GUS_Hyg
Plasmid#159705PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsGUSExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 CLV3
Plasmid#115485PurposeCRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM162
Plasmid#84088Purposetarget miRNA162 for destruction in Arabidopsis thalianaDepositorInsertSTTM162
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEL065
Plasmid#124896PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NGv1)DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 ACT2p
Plasmid#153374PurposePlant promoterDepositorInsertACT2 promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 RbcS2bt
Plasmid#153387PurposePlant terminatorDepositorInsertRbcS2b terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 g4+g17 (FWA)
Plasmid#119672PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAsDepositorInsertg17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only