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Showing: 1551 - 1600 of 3899 results

  4. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

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  5. 5aa SunTag VP64 EVD

    Plasmid
    #115483
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    115483-plasmid-map-sequence-id-238821

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  16. L2-AA012

    Plasmid
    #185751
    Purpose
    Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Inserts
    HygR2
    eGFP
    Use
    Tags
    membrane localization tag Lti6b
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185751-plasmid-map-sequence-id-367862

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  21. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid
    #68223
    Purpose
    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68223-plasmid-map-sequence-id-135584

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  25. pMAP L2Γ

    Plasmid
    #153358
    Purpose
    Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication
    Depositor
    Naomi Nakayama
    Article
    Andreou et al bioRxiv, March 31, 2021
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    153358-plasmid-map-sequence-id-317048

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  26. pXSE901B

    Plasmid
    #91713
    Purpose
    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91713-plasmid-map-sequence-id-180059

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  31. pUPD2:35S-TMV-Rep-MP

    Plasmid
    #106217
    Purpose
    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)
    Depositor
    Tomáš Moravec
    Insert
    Infectious clone of Tobacco mosaic virus, 5' part
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106217-plasmid-map-sequence-id-208883

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  34. pAM-35s-CERK1 D441V-YFPc-1

    Plasmid
    #102403
    Purpose
    split YFP. Plant expression of CERK1 D441V-YFPc-1
    Depositor
    Silke Robatzek
    Insert
    CERK1 (CERK1 Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    D441V
    Promoter
    35S
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    102403-plasmid-map-sequence-id-197600

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  35. pAM-35s-Lyk5-YFPn-1

    Plasmid
    #102389
    Purpose
    split YFP. Plant expression of Lyk5-YFPn-1
    Depositor
    Silke Robatzek
    Insert
    LYK5 (LYK5 Mustard Weed)
    Use
    Tags
    YFPn
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102389-plasmid-map-sequence-id-196611

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  40. pAM-35s-CBL2-YFPc-1

    Plasmid
    #102394
    Purpose
    split YFP. Plant expression of CBL2-YFPc-1
    Depositor
    Silke Robatzek
    Insert
    CBL2 (CBL2 Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102394-plasmid-map-sequence-id-196626

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  45. pYPQ269D

    Plasmid
    #164717
    Purpose
    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    zCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)
    Use
    CRISPR; Gateway compatible pmcda1-cbe_v04 entry c…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164717-plasmid-map-sequence-id-325388

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  48. pMpGWB121

    Plasmid
    #68575
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    MpEF1alpha promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68575-plasmid-map-sequence-id-121822

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  50. pEE495

    Plasmid
    #149291
    Purpose
    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149291-plasmid-map-sequence-id-305898

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Showing: 1551 - 1600 of 3899 results