-
Plasmid#225987PurposeTo make Gateway entry clone of CRISPR guide RNA under AtU6pDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pAGM55285
Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
3TERM_35S
Plasmid#136114Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_35S (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
P427-FBP_13
Plasmid#139704PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/Vn-C
Plasmid#183154PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF101.2
Plasmid#134771PurposeA binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:DsRed:Tnos (GB0361)
Plasmid#68220PurposeTranscriptional unit for DsRed plant expression driven by the 35S promoterDepositorInsertDsRed
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAtC
Plasmid#78098PurposePlant expression of Cas9 and gRNA empty backbone; Hygro ResistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TMEr
Plasmid#61208PurposeFluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-mCherry-BCP
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGC1::YC3.60
Plasmid#107061PurposeExpressing Ca sensor yellow cameloen 3.6 under guard cell specific promoterDepositorInsertYC3.6
UseTagsExpressionPlantMutationPromoterGC1 (At1g22690)Available sinceApril 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHW00016
Plasmid#222447PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.DepositorInsertp65AD-NLS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROF148
Plasmid#155330PurposeBinary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette.DepositorInsertLB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-StUbi-6
Plasmid#197710PurposePhytobrick (MoClo) Level 0 PartDepositorInsertStUbi promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM113
Plasmid#127210PurposeBeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertWUS2, AtSTM, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH102
Plasmid#128207PurposesgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertsgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH-roGFP2-synORP1
Plasmid#191680PurposeTi plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.DepositorInsertrredox(roGFP2-synORP1
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146-ZmUbi-tRNA
Plasmid#158404PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB412
Plasmid#74806PurposeGateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-SlUbi-6
Plasmid#197709PurposePhytobrick (MoClo) Level 0 PartDepositorInsertSlUbi promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-SlUbi-11
Plasmid#197773PurposePhytobrick (MoClo) Level 0 PartDepositorInsertSlUbi terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPEM
Plasmid#162467PurposeDestination Expression vector for monocot plant primer editingDepositorInsertCas9H840A
UseTagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlaninePromotermaize UbiAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT5439
Plasmid#192351PurposeL0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stopDepositorInsertTTCG_Stop-tOCS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT9403
Plasmid#192352PurposeL0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stopDepositorInsertTTCG_Stop-tNOS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH66
Plasmid#131765PurposeLevel1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOStDepositorInsertZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
JAB2240
Plasmid#190359PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-NLS-19Sterm)DepositorInsertproG1090::PhlF-NLS-19Sterm
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX024
Plasmid#126242PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf03035g01006.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version1
Plasmid#197931PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTagsFLAG tag and SV40 NLS and nucleoplasmin NLSExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136B2.0
Plasmid#167160PurposeGolden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 AlcR
Plasmid#153410PurposeEthanol inducible system trans activatorDepositorInsertAlcR CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB415
Plasmid#74809PurposeGateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:GW
Plasmid#132358PurposeExpression of CDS from the Zea mays ubiquitin promotorDepositorTypeEmpty backboneUseTagsYFPExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFBP_2xNbLYS1::LUZ
Plasmid#189918PurposeBinary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.DepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromoterVariousAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb-35S
Plasmid#161008PurposePP7-tagged reporter driven by the 35S promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
35S-DsREDmonomer-NosT
Plasmid#79183PurposeTransient expression vector for DsRED monomerDepositorTypeEmpty backboneUseReporter plasmidTagsDsREDmonomerExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM55297
Plasmid#153213PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-ZmUbi-tRNA
Plasmid#158402PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB408
Plasmid#74802PurposeGateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK-N. tabacum trnI/trnA
Plasmid#123579PurposeMoChlo kitDepositorInsertN. tabacum trnI/trnA integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGX-5Dual
Plasmid#226869Purposetransient expressionDepositorInsertLuciferase and hRluc
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p35S-GW-3Avi
Plasmid#127918PurposeGateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplastDepositorTypeEmpty backboneUseTags3xAviTagExpressionPlantMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2-myc-mCherry
Plasmid#86159PurposeFLS2-mCherry expressionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTagsmCherry and mycExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262-ABE8e
Plasmid#161523PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editingDepositorInsertABE8e-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e-zspcas9(d10a) en…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH103
Plasmid#128208PurposecrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH47
Plasmid#125812PurposeLevel0 LbCas12a, human codon optimizedDepositorInsertLbCas12a, human codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH54
Plasmid#128183PurposeLevel1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3AtDepositorInsertAtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4212
Plasmid#141313PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHairyRed (double 35s removed)
Plasmid#69710PurposeFluorescent reporter of Agrobacterium rhizogenes mediated transformed roots - modifiedDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19/ER-mChe
Plasmid#183163PurposeConstruct for transient expression of endoplasmic reticulum-targeted mCherry in plants.DepositorInsertER-mCherry
UseTags25 aa ER signal of Glycine soja kunitz-type tryps…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM319
Plasmid#84201PurposeDisturb the expression of miR319 in riceDepositorInsertmiR319
UseTagsExpressionBacterial, Plant, and Yeast…MutationPromoter2x35SAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only