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Plasmid#136238PurposeProtein expression of Beta-galactosidase with C-terminal V5 and His tagsDepositorInsertBeta-galactosidase
UseTagsHis and V5ExpressionInsectMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster U6:3Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster nanosAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pActin5c-CD-dCas9m4-UDI-attB
Plasmid#104879PurposeTo generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9DepositorInsertCD-dCas9(m4)-UDI
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-WT
Plasmid#114063Purposeprotein productionDepositorInsertMps1-WT (TTK Human)
UseTagsHis6ExpressionInsectMutationnonePromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-YFPsv40 GGGG AAGA
Plasmid#174530Purpose3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-YFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Consensus
Plasmid#176664PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-EGFP-Actin5c
Plasmid#15312DepositorInsertActin5c (Act5C Fly)
UseTagsEGFP and HisExpressionInsectMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pKM19
Plasmid#123655PurposeEncodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cellsDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionInsectMutationPromoterpIExAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT Lifeact-TagBFP
Plasmid#126664PurposeInducible expression of Lifeact-TagBFP in S2 cellsDepositorInsertLifeact-TagBFP fusion
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
gp64-mVEGF-C-HisTag-pENTR
Plasmid#64065PurposePlasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.DepositorInsertvascular endothelial growth factor C (Vegfc Mouse)
UseTagsHis-tagExpressionInsectMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-QS
Plasmid#24341DepositorInsertQS
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pIZ-Flag6His-Siwi
Plasmid#50560PurposeExpresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cellsDepositorInsertSiwi (Aubergine )
UseTags6xHIS and FLAGExpressionInsectMutationPromoterOpIE2Available sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto Grx1-roGFP2
Plasmid#64994Purposefor expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophilaDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3GFP
Plasmid#182205Purpose3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
DmU6 3p3 GFP LgRNA
Plasmid#182207PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in DrosophilaDepositorInsert3XP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAC-ECFP-15xQUAS_TATA-mcd8-GFP-SV40
Plasmid#104878PurposepiggyBac vector of 15xQUAS-mCD8:GFP; used for mosquito tranformationDepositorInsertmCD8:GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only