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Showing: 621 - 640 of 4645 results

  4. DPYSL4 (DPYSL4A-c003)

    Plasmid
    #110263
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPYSL4 (DPYSL4 Human)
    Use
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110263-plasmid-map-sequence-id-212341

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  9. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid
    #22017
    Purpose
    Expression of photoactivatable Rac1 (dominant-negative mutant)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    Use
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutation T17N.
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22017-plasmid-map-sequence-id-138512

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  13. p2Bac.His.XPB

    Plasmid
    #39216
    Depositor
    Aziz Sancar
    Insert
    XPB (ERCC3 Human)
    Use
    Tags
    His
    Expression
    Insect
    Mutation
    Promoter
    Pph
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  14. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Use
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

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  16. CDK12

    Plasmid
    #73258
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDK12 (CDK12 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    pfam00069: Pkinase 727-1020
    Promoter
    Polyhedrin
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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Showing: 621 - 640 of 4645 results