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Plasmid#83380PurposeDrosophila gRNA expression plasmid targets ebonyDepositorInsertebony (e Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL-ubi63E
Plasmid#74280PurposeRenilla control plasmid driven by the ubiquitin 63E promoterDepositorInsertRenilla luciferase
UseTagsExpressionInsectMutationPromoterubiquitin 63E promoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTwt full-length
Plasmid#109161PurposeEncodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length REST/NRSF (REST Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSSRB069_pAC8-hsDDB1dB
Plasmid#218790PurposeInsect cell expression vector for 6xHis-TEV-hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTags6xHis-TEVExpressionInsectMutationDeleted amino acids 396-705 and replaced with GNG…PromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p10xQUAS-CsChrimson
Plasmid#163629Purpose10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequenceDepositorInsertCsChrimson.mVenus
UseTagsmVenusExpressionInsectMutationPromoterAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL
Plasmid#83342Purposefor Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectMutationPromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-puro
Plasmid#208031PurposeEnables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTagsEGFPExpressionInsectMutationPromoterhsp70Available sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct-PE2-HygroR
Plasmid#149552PurposeExpression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.DepositorInsertPE2
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGE-attBTT-loxP-DsRed (pTL780)
Plasmid#115160PurposeIntegration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.DepositorInsertattB-TT loxP 3xP3-DsRed
UseCre/LoxTagsExpressionInsectMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-MZT1
Plasmid#178075PurposeInsect cell expression of MZT1DepositorInsertMZT1 (MZT1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCFD3-NS
Plasmid#149545PurposeExpression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-GFP-V5-His
Plasmid#85621PurposeExpresses GFP and V5 in Drosophila.DepositorInsertGFP
UseTagsV5, HisExpressionInsectMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQUASp-mCD8mCherry
Plasmid#46164PurposeExpression of murine CD8mCherry in insectsDepositorInsertmCD8mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tubp-Gal4 in pCaSpeR4
Plasmid#17747DepositorInsertGAL4 (GAL4 Budding Yeast)
UseTagsTubulin 1alpha promoter and hsp70 PolyAExpressionInsectMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
UseTagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only