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Plasmid#174480PurposeExpresses DDA1 in insect cellsDepositorInsertDDA1 (DDA1 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx-mVenus-PA-Rac1
Plasmid#22007PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseTags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_641-1574_AH
Plasmid#148920PurposeInsect Expression of DmGIGYF_641-1574DepositorInsertDmGIGYF_641-1574 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_1-640_AH
Plasmid#148919PurposeInsect Expression of DmGIGYF_1-640DepositorInsertDmGIGYF_1-640 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-7-QFBDAD-hsp70
Plasmid#46115DepositorInsertQF#7
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINE
Plasmid#26043DepositorInsertHis-lacZ-KHHHHHH
UseTagsKHHHHHH and MAHHHHHHSSGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-sfGFP-3xP3-RFP
Plasmid#127557PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertsfGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOCC151
Plasmid#118899Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3CDepositorInsertNcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsGST tag, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K963_GFP_SNAP
Plasmid#159727PurposeExpresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-LexAGAD)
Plasmid#194769PurposeTo insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.DepositorInsertT2A-LexAGAD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNDamMyc
Plasmid#59217PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.DepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectMutationPromoterDrosophila heat-shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC82
Plasmid#118862Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant proteinDepositorInsertNcoI-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAS-myr::4xSNAPf
Plasmid#87637PurposeFor creating drosophila transgenics expressing UAS-myr4x::SNAPfDepositorInsertmyr::4xSNAPf
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3xVHH05-DsRed
Plasmid#171581PurposeFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only