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mTubulin NLS-mCherry-LEXY

Plasmid

#182597

Purpose

Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

mCherry

Use

Tags

LEXY and NLS

Expression

Insect

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EIF3B:127-814

Plasmid

#210909

Purpose

Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:D127-E814 (EIF3B Human)

Use

Tags

MHHHHHHSSGRENLYFQGMQ

Expression

Insect

Mutation

Y760C sequence conflict

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pOCC153

Plasmid

#118901

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

TwinStrep, cleavable with 3C protease

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N116C-G193C

Plasmid

#162580

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Asparagine 116 was mutated to Cysteine and Glycin…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINM

Plasmid

#26044

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His-MBP-3C-lacZ

Use

Tags

MAHHHHHHSSG_MBP_LEVLFQGP

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pJFRC164-21XUAS-KDRT>-dSTOP-KDRT>-myr::RFP

Plasmid

#32141

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

KDRT> STOP STOP KDRT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

Plasmid

#48295

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

His6

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP1

Plasmid

#59036

Purpose

for baculovirus expression of CAMSAP1 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 (CAMSAP1 Human)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pJFRC18-8XLexAop2-mCD8::GFP

Plasmid

#26225

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

mCD8::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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15x-QUAS-dTomato-T2A-GCaMP6s

Plasmid

#130666

Purpose

QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

Depositor

Leslie Vosshall

Article

Matthews et al Elife. 2019 May 21;8. pii: 43963. doi: 1

Insert

15xQUAS-dTomato-T2A-GCaMP6s

Use

Ae. aegypti expression

Tags

Expression

Insect

Mutation

Promoter

15xQUAS-TATA

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC2-10XUAS-IVS-mCD8::GFP

Plasmid

#26214

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

mCD8::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor Q63L (RHOA Human)

Use

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAcGHLT-B-DDB1

Plasmid

#48638

Purpose

Expresses Full Lenght DDB1 in Insect Cells (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

DDB1 (DDB1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
pE2n

Plasmid

#17456

Purpose

modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2::VP16Uw

Plasmid

#26228

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

GAL4::VP16 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pUAST_NSlmb-vhhGFP4

Plasmid

#35575

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pUASTattB_NSlmb-vhhGFP4

Plasmid

#35577

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge