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Plasmid#193058PurposeBaculovirus expression of S.pombe Fml1 for recombinant protein productionDepositorInsertFml1 (fml1 Fission Yeast)
UseTags3xFlagExpressionInsectMutationPromoterpolyhedronAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFlpStop-attB-UAS-2.1-tdTom
Plasmid#88910PurposeattB FlpStop plasmid, for insertion into MiMIC siteDepositorInsertFlpStop
UseTagsExpressionInsectMutationPromoter5XUASAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P10.1-StrepTagII
Plasmid#191475PurposeExpresses K2P10.1 with C-terminal StrepTagIIDepositorUseTagsExpressionInsectMutationN144Q (uniprot P57789-1)PromoterpolHAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_3xFLAG
Plasmid#101654PurposeExpression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, 3xFLAG tag
UseTags3xFLAG tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2
Plasmid#125161PurposeExpresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertEZH2 (EZH2 Human)
UseBaculovirus expression vectorTagsHis-MBPExpressionInsectMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EED
Plasmid#125163PurposeExpresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EYFP,attP}
Plasmid#86860PurposeExpresses EYFP in eyes and contains an attP landing site for site-specific attB integrationDepositorInsertattP
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.JARID2
Plasmid#125166PurposeExpresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertJARID2 (JARID2 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR TPP1 MBP TEV TIN2
Plasmid#185446PurposeCoexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 WT
Plasmid#110127PurposeExpresses Flag-tagged human URH49 (DDX39A)DepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceApril 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
10xUAS-GFP-DD
Plasmid#104989PurposeExpresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized GFP (GFP-DD)
UseTagsDDExpressionInsectMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-syt1-GCaMP6f
Plasmid#190896PurposeGCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.DepositorInsertsyt1-GCaMP6f
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
Plasmid#115694PurposeAll-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.DepositorInsertsNmeCas9
Nme-sgRNA
UseCRISPRTagsNLS and NLS, HAExpressionInsect and MammalianMutationNone and fusion of repeat/tracrRNA to make a sgRNAPromoterEF-1 alpha and U6Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
UseTags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
UseTagsV5ExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PSL1180polyUBdsRED
Plasmid#49327PurposeDonor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertdsRED
UseTagsExpressionInsectMutationPromoterAedes polyubiquitinAvailable sinceNov. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac LIC cloning vector (4A)
Plasmid#30111DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only