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Plasmid#37676DepositorInsertHhN
UseLuciferaseTagsrenilla luciferaseExpressionInsectMutationPromoterActin 5CAvailable sinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASt-FMW-attB-AthHen1
Plasmid#104958PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 driversDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterHsp70 promoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CG4845/pUAST
Plasmid#17588DepositorInsertCG4845 (psidin Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
57F03_GAL80
Plasmid#83254PurposeExpresses GAL80 under the control of 57F03 enhancerDepositorInsert57F03 enhancer
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPgCdH3
Plasmid#8695DepositorInsertcid promoter, H3 (His3:CG31613 Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
pattB-3xFlag-Loqs-PB
Plasmid#41109DepositorInsertLoquacious-PB (loqs Fly)
UseTags3xFlagExpressionInsectMutationPromoterAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKF296
Plasmid#74773Purposetemplate for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-ResistanceDepositorInsertTEV- 2xV5 tag (short version)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRB32
Plasmid#72863Purposetemplate for N-terminal 1x Strep tag, constitutive Blasticidin resistanceDepositorInsertsingle Strep tag
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A PH+DH domains
Plasmid#69793PurposePebble isoform A, PH and DH domains, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH +PH domains (pbl Fly)
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationPbl-A (NP_729306.1) amino acids 385-647.Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
LexAop2->Bxb1.STOP>myr::4xSNAPf
Plasmid#87640PurposeFor creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPfDepositorInsertmyr::4xSNAPf
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PI(DM)-Rac1(WT)dc1.FLARE
Plasmid#87589PurposePI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
UseTagsYPet and dTurqExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FoRC.PRm
Plasmid#84587PurposeDrosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASP Flag Rab32 WT
Plasmid#44833DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsFlagExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pMCh-139
Plasmid#92287PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cellsDepositorInsertdGW182 NED (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 NED: aa 205-490PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 WT
Plasmid#58444Purposeexpression of drosophila YFP-Rab2DepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-NipB
Plasmid#71502PurposeflySTARR-seq screening vector with NipB core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab32 DN
Plasmid#37739DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT33N (dominant negative)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pattB-aubergine-ADH-gf
Plasmid#69448Purposegenomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead AubDepositorInsertaubergine (aub Fly)
UseTagsNAExpressionInsectMutationchr2L:10,998,626(−)T>GPromoteraubergineAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only