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pCIOX

Plasmid

#51300

Purpose

E.coli expression vector for His8-SUMO tagged recombinant protein

Depositor

Andrea Mattevi

Type

Empty backbone

Use

Tags

His8 + SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTet

Plasmid

#124663

Purpose

Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTet

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a-AldoA

Plasmid

#224925

Purpose

Inducible expression of human aldolase A

Depositor

Liskin Swint-Kruse

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

Aldolase A (ALDOA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-P_vanR-PVanCC

Plasmid

#204078

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

vanR repressor and PVanCC vanillic acid-inducible promoter

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAGE2.0-i

Plasmid

#194639

Purpose

Empty pAGE2.0 plasmid with pBAD inducible promoter

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/CURT_fluoA

Plasmid

#117998

Purpose

Expression of CURT_fluoA controlled by the ARA promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoA

Use

Tags

Expression

Bacterial

Mutation

Promoter

ARA

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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MP-S2

Plasmid

#69691

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 AAG(Y127I-H136L)

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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MP-S3

Plasmid

#69692

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 d80-AAG(Y127I-H136L)

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAQ_G-alpha

Plasmid

#109809

Purpose

Protein expression and purification of GNAQ_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAQ_G-alpha (GNAQ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-PA-Rac1

Plasmid

#22007

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Tags

6X His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP

Plasmid

#134357

Purpose

Reporter plasmid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Inserts

GFP
mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-His6-1_HBD

Plasmid

#171869

Purpose

Expresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Hybrid binding domain of Rnase H1

Use

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pQLinkN

Plasmid

#13670

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

Insert

multi cloning site

Use

Co-expression

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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RP hk339-GFP

Plasmid

#24431

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin WT (KIF5B Human)

Use

Tags

6XHis and GFP

Expression

Bacterial

Mutation

monomeric kinesin

Promoter

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only

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pROP-IP-GFP

Plasmid

#122136

Purpose

GFP Reporter plasmid for B-Rex contain imperfect operator sequence

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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ScSpe1 pET-20b(+)

Plasmid

#117145

Purpose

Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

Depositor

Markus Ralser

Article

Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

Insert

SPE1

Use

Tags

Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9cur

Plasmid

#71538

Purpose

Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Inserts

Cas9
Plasmid curing system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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