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Plasmid#140084PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pROP-IP-FBFP
Plasmid#122138PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)DepositorInsertFBFP
UseTagsExpressionBacterialMutationPromoterJ23101Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mCherry
Plasmid#199247PurposeFluorescent labelling of bacterial cells with mCherryDepositorInsertCodon-optimized mCherry gene
UseTagsExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEERM4 Cm
Plasmid#64026PurposePlasmid for integration into neutral site 2 of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis T4 DNA Ligase
Plasmid#206207PurposeInducible expression of T4 DNA ligaseDepositorInsertT4 DNA ligase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-uvsY
Plasmid#163914PurposeExpresses uvsY for bacterial expression and affinity purificationDepositorInsertuvsY (uvsY Enterobacteria phage T4)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b His-p97
Plasmid#169020PurposeExpresses His-p97 in bacteriaDepositorInsertp97
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.3
Plasmid#42632DepositorInsertHuman H3.3 (H3-3A Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Cas9-6xHis
Plasmid#62374PurposeExpression of Cas9-6xHis in bacterial cellsDepositorInsertCas9_6xHis
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSFlp
Plasmid#45470DepositorInsertFlp recombinase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pM1s3ATScsg-Etag
Plasmid#137946PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expressionDepositorInsertsSynthetic curli operon
Synthetic curli operon
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterpTlpA36Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
5WCI (MYST1)
Plasmid#101269PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMYST1 (KAT8 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-human-p16INK4A-P2A-mCherry
Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
UseTagsP2A-mCherryExpressionBacterialMutationPromoterno promoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone76)
Plasmid#188579PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
UseTagsExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU1_000
Plasmid#186773Purpose* Engineered U1 snRNA plasmid for insertion of guide sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU1 PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-uvsX-H6
Plasmid#163913PurposeExpresses uvsX for bacterial expression and affinity purificationDepositorInsertuvsX
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-590
Plasmid#38314DepositorInsert5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv2
Plasmid#188604PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only