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Plasmid#164901PurposeThe pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.DepositorInsertphi21 Integrase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC
Plasmid#99641PurposeBacterial expression of wildtype Rag GTPase dimerDepositorUseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS*11_4enh
Plasmid#149423PurposePlasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).DepositorInsertsrbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer
UseTagsT120>AExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-CalfluxVTN
Plasmid#83926PurposeBioluminescent Reporter of Intracellular CalciumDepositorInsertCalfluxVTN
UseTagsExpressionBacterialMutationNonePromoterT7Available sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR2F2_Hormone-recep
Plasmid#109826PurposeProtein expression and purification of NR2F2_Hormone-recepDepositorInsertNR2F2_Hormone-recep (NR2F2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptet-sfGFP
Plasmid#139935PurposeaTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertTetR-Ptet-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-EGFP-HaloTag2
Plasmid#41742DepositorInsertHalotag 2
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen
Plasmid#182413PurposeBacterial expression of mNeongreenDepositorInsertmNeongreen
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6, LgBiTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJOE3075.3
Plasmid#135084PurposeL-rhamnose-inducible expression vectorDepositorInsertP-rhaP-BAD-malE
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterJ231196Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDiGi
Plasmid#59323PurposeInducible expression of dsRed and GFP in bacteriaDepositorInsertsEGFP
dsRed
UseTagsExpressionBacterialMutationPromoterPbad and PlacAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTP1112
Plasmid#104158PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_DNAseI
Plasmid#182408PurposeBacterial expression of DNaseIDepositorInsertDNase I (DNASE1 Bovine)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL3
Plasmid#67376PurposeExpression of native hArl3 in E. coliDepositorInsertARL3 (ARL3 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
UseTagsExpressionBacterialMutationPromoterPosmY, PsulA, and grpEAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oDi
Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM660
Plasmid#68889PurposeArabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only