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Plasmid#122652PurposeExpression of hFerritinH in bacterial cellsDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-6P-1 SARS-CoV 3CLpro
Plasmid#61692Purposeinducible expression of SARS 3CLpro proteaseDepositorInsertSARS 3CLpro protease
UseTagsGST and HISExpressionBacterialMutationPromotertacAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSC5
Plasmid#188602PurposeSuperior Conjugative Plasmid to Diverse YeastDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC011
Plasmid#171143PurposeExpression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmidDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIhsp+LuxG13
Plasmid#49999PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertBacterial luciferase operon + G13 promoter
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterG13 from M. marinum G13 promoter driving luxCAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15-MHL
Plasmid#26092PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneUseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAM5085
Plasmid#85122PurposePkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.DepositorInsertPkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
UseTagsECFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP8045
Plasmid#220900PurposeTn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroidesDepositorInserttnsABCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYC01
Plasmid#224586PurposeIPTG-inducible phage φX174 lysis gene EDepositorInsertphage φX174 lysis gene E (phiX174p08 )
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPA1lacO-1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTwt
Plasmid#207466PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorInsertTdTwt (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Lime
Plasmid#191255PurposeBacterial expression plasmids for pH sensitive fluorescent protein LimeDepositorInsertLime
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Artemis
Plasmid#40211PurposeMammalian expression of codon-optimized Artemis.DepositorInsertArtemis (DCLRE1C Human)
UseTagsExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp7-His
Plasmid#11825DepositorInsertCaspase 7 (CASP7 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-sfGFP
Plasmid#163757PurposeExpresses Streptomyces codon-optimised sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterSP44Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen
Plasmid#182387PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen fusionDepositorInsertVNp-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_RFP-SZ2
Plasmid#124933PurposeExpression of RFP-SZ2DepositorInsertRFP-SYNZIP2
UseTagsHis and mCherryExpressionBacterialMutationSZ2 domainPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only