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pCDM4

Plasmid

#49796

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pACM4

Plasmid

#49797

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pCOM4

Plasmid

#49798

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM3

Plasmid

#49799

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDM4-GLY

Plasmid

#49808

Purpose

Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

phosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pACM4-ACA

Plasmid

#49809

Purpose

Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

ACP transacylase
Acetyl-CoA carboxylase
acetyl-CoA carboxylase
Acetyl-CoA carboxylase
Acetyl-CoA carboxylase

Use

Tags

Expression

Bacterial

Mutation

Silent site-directed mutagenesis to remove SalI s…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pETM6-FAS

Plasmid

#49810

Purpose

Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

fatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase

Use

Tags

Expression

Bacterial

Mutation

Codon-optmized. See supplemental information of …

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlink.a1-236

Plasmid

#49813

Purpose

expression vector for MBP-SRAP(1-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Use

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlnk.a13-236

Plasmid

#49814

Purpose

expression vector for MBP-SRAP(13-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Use

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
hSRAP.TWIN1.a13-69

Plasmid

#49815

Purpose

expression vector for SRAP(13-69) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-69 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
hSRAP.TWIN1.a13-87

Plasmid

#49816

Purpose

expression plasmid for SRAP(13-87) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-87 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
hSRAP.TWIN1.a13-215

Plasmid

#49817

Purpose

expression vector for SRAP(13-215) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-215 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a105-215.V105M

Plasmid

#49818

Purpose

expression vector for SRAP(105-215)V105M in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 105-215 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

V105M codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a105-236.V105M

Plasmid

#49819

Purpose

expression vector for SRAP(105-236)V105M in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 105-236 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

V105M codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
hSRAP.TWIN1.a111-215

Plasmid

#49820

Purpose

expression vector for SRAP(111-215) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 111-215 (SRA1 Human)

Use

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
SLIRP.HMG6.wt.fl

Plasmid

#49821

Purpose

expression vector for MBP-SLIRP fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)

Use

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
SRAcode_in_pUC57_v3

Plasmid

#49822

Purpose

for transcribing SRA nucl 419-1356 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Use

Tags

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
SRAcode_in_pUC57_v3(419-771)

Plasmid

#49823

Purpose

for transcribing SRA nucl 419-771 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Use

Tags

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
SRAcode_in_pUC57_v3(419-999)

Plasmid

#49824

Purpose

for transcribing SRA nucl 419-999 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Use

Tags

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
SRAcode_in_pUC57_v3(767-999)

Plasmid

#49825

Purpose

for transcribing SRA nucl 767-999 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Use

Tags

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge