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Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
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pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
UseTagsExpressionBacterialMutationPromoterglnS' and lambda(ts) and proKAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DNAseI
Plasmid#182409PurposeBacterial expression of Vesicle Nucleating peptide-DNaseI fusionDepositorInsertVNp-DNase I (DNASE1 Bovine)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ClpP-his
Plasmid#22144DepositorInsertClpP-His6
UseTagshisExpressionBacterialMutationC-terminal His tagPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-mOVA
Plasmid#25099DepositorInsertmembrane bound ovalbumin
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
UseTagsHisExpressionBacterialMutationE54K and L372PPromoterAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-tag (T7) Vector
Plasmid#101137PurposeExpression of protein of interest with SNAP-Tag in bacterial cellsDepositorTypeEmpty backboneUseTagsSNAP-tag (SNAP26b)ExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET his-eGFP
Plasmid#113551PurposeFor bacterial expression and purification of eGFPDepositorInserthis-eGFP
UseTagsExpressionBacterialMutationA206KPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa
Plasmid#22163DepositorInserthuman Dynamin 1aa (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationD744NPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pC009 LshCas13a locus with targeting spacer
Plasmid#91900PurposeLshCas13a locus into pACYC184 with targeting spacerDepositorInsertLshCas13a locus with targeting spacer
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNF02-mLemon
Plasmid#219399PurposeConstitutive expression of mLemonin E. coliDepositorInsertmLemon
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF-HdrB3
Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBR322-PVCpnf17-22
Plasmid#198272PurposeUnmodified pPayload; contains WT PVC payloads/regulatory genesDepositorInsertPVCpnf17-22
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAKgfp1
Plasmid#14076DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9- AsiA_m2.1
Plasmid#158065PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 2.1DepositorInsertdCas9-AsiA_m2.1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MazF-WT
Plasmid#155202PurposeTo express recombinant MazF protein.DepositorInsertMazF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLinkH
Plasmid#13667DepositorInsertmulti cloning site
UseCo-expressionTagsHis, TEV, and attB1ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only