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Plasmid#111467Purposetranslational elongation factor TsDepositorInserttranslational elongation factor Ts
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B7-1
Plasmid#111489Purposetranslational elongation factor GDepositorInserttranslational elongation factor G
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-fis
Plasmid#51557PurposeE. coli fis ORF cloned into pBAD24 expression vectorDepositorInsertFIS (fis E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
M13cp-dg3 hp
Plasmid#218095PurposeHelper plasmid that allows phagemid production upon complementation with a phagemid expressing pIIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
B5-5
Plasmid#111484Purposetranslational initiation factor 3DepositorInserttranslational initiation factor 3
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B3-1
Plasmid#111471Purposetranslational initiation factor 2DepositorInserttranslational initiation factor 2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-5
Plasmid#111479Purposeribosome recycling factorDepositorInsertribosome recycling factor
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMMB66EH-aphA
Plasmid#44392DepositorInsertAphA
UseTagsExpressionBacterialMutationPromotertacAvailable sinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pCR8-attL1-pegRNA-attR3
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBP-MS2
Plasmid#65104Purposecan be used to isolate RNA binding complexesDepositorInsertMS2
UseTagsMBPExpressionBacterialMutationdouble mutation (V75Q and A81G) that prevents oli…PromotertacAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCM291rfp
Plasmid#59775PurposepCMrfp with TrfA mutation (291E to G)DepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(C163S)
Plasmid#90088PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationC163SPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLS.405
Plasmid#187203PurposeExpresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA (wildtype)
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFKM1
Plasmid#64950Purposesource of KmR FRT cassetteDepositorInsertFRT-KmR-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_5+7_gfp
Plasmid#60595Purposeencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.DepositorInsertCas_5+7_gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_2+5
Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
STK24_HUMAN_D0
Plasmid#79742PurposeThis plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK24 (STK24 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-distrepII
Plasmid#196667PurposeExpression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only