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Addgene
Showing: 10181 - 10200 of 27133 results

  3. pAM5080

    Plasmid
    #85106
    Purpose
    Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85106-plasmid-map-sequence-id-189868

    Enlarge

  4. pAM5081

    Plasmid
    #85107
    Purpose
    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    YFP
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85107-plasmid-map-sequence-id-180104

    Enlarge

  13. pAM5082

    Plasmid
    #85121
    Purpose
    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiB-KaiC-ECFP
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85121-plasmid-map-sequence-id-180106

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  14. pAM5085

    Plasmid
    #85122
    Purpose
    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85122-plasmid-map-sequence-id-180108

    Enlarge

  20. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

    Enlarge

Showing: 10181 - 10200 of 27133 results