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Plasmid#13423DepositorInsertBA
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI
Plasmid#44182DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
IVT-MCP-MMLV-RT
Plasmid#207457Purposein vitro transcription of mRNADepositorInsertMCP-RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRuby-pBAD
Plasmid#54763PurposeLocalization: Protein Expression Vector , Excitation: 558, Emission: 605DepositorTypeEmpty backboneUseTags6xHis and mRubyExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
UseTagsMBP, hexa His, and two SV40 NLSExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-6h-GST-TEVp
Plasmid#172887PurposeCodon optimized (E. coli) TEVp fused with GST and his-tagged for purificationDepositorInsertTEV protease
UseTagsGST solubility tag and HHHHHHExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
As4
Plasmid#123161PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-tDepositorInsertJ115-30arsR-t-ParsR-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ115, ParsRAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
Plasmid#110201PurposeMelanocyte-specific expression of the LexPR-2A-Cerulean fusionDepositorInsertLexPR transactivator - 2A - Cerulean
UseTagsCeruleanExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA5-lenti
Plasmid#115664PurposeExpresses AcrVA5 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA5
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD
Plasmid#168153PurposeInducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pEGFPN1-human Dynamin 1aa K44A
Plasmid#22197DepositorInserthuman Dynamin 1aa K44A (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
UseTagsExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109Hg-gfp
Plasmid#123148PurposeA mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerTAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ma-Barnase
Plasmid#197572PurposeNegative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertsBarnase - 2xTAG
M. alvus Pyl-tRNA (6)
UseTagsnoneExpressionBacterialMutation3TAG and 45TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only