Showing: 7981 - 8000 of 27125 results
-
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
UseTagsExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
UseTagsExpressionBacterialMutationPromoterlac UV5AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)AvailabilityAcademic Institutions and Nonprofits only -
pKSII/INSeGFP
Plasmid#53787Purpose1 kb zebrafish insulin promoter driving eGFPDepositorInsertINS promoter/eGFP/pA
UseZebrafishTagseGFPExpressionBacterialMutationPromoterzebrafish insulin/ZIPAvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-Ins2-842
Plasmid#53969Purpose842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assaysDepositorInsertmouse Ins2 gene partial (Ins2 Mouse)
UseTagsExpressionBacterialMutationPromoterlacAvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-INS-904
Plasmid#53970Purpose904 bp insert from human INS gene used as a control for methylation-specific PCR assaysDepositorInserthuman INS gene partial (INS Human)
UseTagsExpressionBacterialMutationPromoterlacAvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/hGGA1(GAT)
Plasmid#79435PurposeExpresses GST-tagged GAT domain of GGA1 (141-327 aa) in E.coliDepositorInsertGGA1 (GGA1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 141-327PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/hGGA3(GAT)
Plasmid#79436PurposeExpresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coliDepositorInsertGGA3 (GGA3 Human)
UseTagsGSTExpressionBacterialMutationamino acids 107-286PromoterAvailabilityAcademic Institutions and Nonprofits only -
5CZG (Tb427.10.7420)
Plasmid#79517PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTb427.10.7420
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5E16 (PfPKG, PF14_0346)
Plasmid#79518PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPF14_0346
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5FAI (EMG1)
Plasmid#79519PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEMG1 (EMG1 Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5FEA (LdBRD19, LdBPK_363520.1)
Plasmid#79520PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLdBPK_363520.1
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5H9M (SIAH2)
Plasmid#79521PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSIAH2 (SIAH2 Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5I3L (DPF3)
Plasmid#79522PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDPF3 (DPF3 Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5J39 (TDRKH)
Plasmid#79523PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTDRD2 (TDRKH Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5J3E (THYN1)
Plasmid#79524PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTHYN1 (THYN1 Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
5J3U (TgPKA, 583.m05422)
Plasmid#79525PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert583.m05422
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
pET28-MKH8SUMO
Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneUseTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v1
Plasmid#79537PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 1DepositorInsertAmrose
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v2
Plasmid#79538PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 2DepositorInsertAmrose v2
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 7981 - 8000 of 27125 results