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Showing: 61 - 80 of 28105 results

  1. pOPO717

    Plasmid
    #195302
    Purpose
    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and lacZ spacer 5 in native array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195302-plasmid-map-sequence-id-383138

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  2. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  3. 6ASB (FBXL19)

    Plasmid
    #119954
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    FBXL19 (FBXL19 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 31-153
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    119954-plasmid-map-sequence-id-232625

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  4. 5W9Q (MBD1)

    Plasmid
    #101265
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD1 (MBD1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101265-plasmid-map-sequence-id-193498

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  5. 4Z3C (TET3)

    Plasmid
    #119969
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    TET3 (TET3 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 48-98
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    119969-plasmid-map-sequence-id-232693

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  6. pAM5082

    Plasmid
    #85121
    Purpose
    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiB-KaiC-ECFP
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85121-plasmid-map-sequence-id-180106

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  9. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Use
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  11. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

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  12. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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  13. pBsVchCAST

    Plasmid
    #203812
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203812-plasmid-map-sequence-id-403828

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  14. pAM5080

    Plasmid
    #85106
    Purpose
    Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85106-plasmid-map-sequence-id-189868

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  20. BirA in pET28a (w400-2)

    Plasmid
    #26624
    Depositor
    Eric Campeau
    Insert
    BirA
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    26624-plasmid-map-sequence-id-228306

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Showing: 61 - 80 of 28105 results