Skip to main content
Addgene
Showing: 761 - 780 of 28122 results

  1. pCas12f1

    Plasmid
    #171182
    Purpose
    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1
    Depositor
    Kohsuke Honda
    Article
    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124
    Insert
    Cas12f1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cas9 promoter from Streptococcus pyogenes
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171182-plasmid-map-sequence-id-336407

    Enlarge

  2. pCDB179-DuraPETase

    Plasmid
    #218699
    Purpose
    Encodes codon optimized DuraPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    DuraPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218699-plasmid-map-sequence-id-434102

    Enlarge

  10. pFC330

    Plasmid
    #87842
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    pyrG
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87842-plasmid-map-sequence-id-189665

    Enlarge

  12. mRuby-pBAD

    Plasmid
    #54763
    Purpose
    Localization: Protein Expression Vector , Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and mRuby
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pNO286-3

    Plasmid
    #107746
    Purpose
    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)
    Depositor
    Jeffrey Tabor
    Article
    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb
    Inserts
    CcaS
    Ho1
    PcyA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Deleted AA 223 and 512
    Promoter
    J23106 and J23108
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107746-plasmid-map-sequence-id-209202

    Enlarge

  14. pBADTrfp

    Plasmid
    #99382
    Purpose
    This promoter system provided the highest RFP expression upon induction
    Depositor
    Nathan Hillson
    Insert
    RFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99382-plasmid-map-sequence-id-192484

    Enlarge

  16. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Use
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

    Enlarge

  18. pEVOL-AcKRS-CloDF

    Plasmid
    #127415
    Purpose
    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127415-plasmid-map-sequence-id-250212

    Enlarge

  19. As4

    Plasmid
    #123161
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J115-30arsR-t-ParsR-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J115, ParsR
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123161-plasmid-map-sequence-id-243984

    Enlarge

Showing: 761 - 780 of 28122 results