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Plasmid#54608PurposeLocalization: Protein Expression Vector , Excitation: 558, Emission: 583DepositorTypeEmpty backboneUseTags6xHis and DsRed2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits
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mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2H12
Plasmid#221142PurposecdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)DepositorInsertcdGreen
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6s
Plasmid#62812PurposepPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4
Plasmid#26103PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis - TEVExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYHY1
Plasmid#215926PurposeE. coli-Bacteroides shuttle vector.DepositorTypeEmpty backboneUseSynthetic Biology; E. coli-bacteroides shuttle ve…TagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-Cas9-His
Plasmid#47327PurposeFor in vitro expression and purification of Cas9 proteinDepositorInsertCas9
UseCRISPRTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLG003 (Druantia type I)
Plasmid#157881PurposeExpresses the Druantia type I defense systemDepositorInsertDruantia type I
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1
Plasmid#79873PurposeBacillus subtilis dCas9 expression vector; integrates into lacA/ganADepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterxylAAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHyo182
Plasmid#173136PurposeExpresses pheS_A294G in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP2
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.661
Plasmid#108532PurposeTtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.DepositorInsertTtgRAM + PTtg-YFP
UseTagsExpressionBacterialMutationTtgR: R130H A144T A148VPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only