Showing: 6421 - 6440 of 27103 results
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Plasmid#80124PurposeI3-01p-myc-Late1-PH (1wa3)DepositorInsertEPN-25 (1wa3)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKT3Ts-goldyTALEN
Plasmid#80330PurposeUpdated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequenceDepositorTypeEmpty backboneUseTALENTagsExpressionBacterialMutationPromoterT3AvailabilityAcademic Institutions and Nonprofits only -
pBAD-smURFP-RBS-HO-1
Plasmid#80341PurposeExpresses smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertssmURFP
HO-1
UseTags6 Histidine TagExpressionBacterialMutationPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pBAD-TDsmURFP-RBS-HO-1
Plasmid#80342PurposeExpresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertsTandem Dimer smURFP
HO-1
UseTags6 Histidine TagExpressionBacterialMutationPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pMALac-OMA-1(1-182)
Plasmid#80350PurposeExpresses OMA-1 (1-182) in bacteria for protein purification purposesDepositorInsertOMA-1 (oma-1 Nematode)
UseTags6xHis-MBP-TEVExpressionBacterialMutationEncodes AA residues 1-182PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorTagsExpressionBacterialMutationPromotercat promoter and cpcG2Δ59AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialMutationPromoterccaR and cpcG2Δ59AvailabilityAcademic Institutions and Nonprofits only -
pVH028
Plasmid#80393PurposeContains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)DepositorUseSynthetic BiologyTagsFused by GS linkers to 4 SH3 domainsExpressionBacterialMutationGlycine 448 changed to Alanine, makes it primaril…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH001
Plasmid#80394PurposeContains aTc inducible Scaffold-mCherry-AAV and TetR genesDepositorInsertmCherry RFP
UseSynthetic BiologyTagsFused by GS linkers to Sh3pep-LZX scaffold protei…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH011
Plasmid#80395PurposeContains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry and TetR genes. Used for Western blots.DepositorInsertBicistronic Scaffold-3xFLAG-SD-mCherry
UseSynthetic BiologyTags3xFLAG tag on scaffold protein for WesternExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH002
Plasmid#80396PurposepTet-Scaffold with no fluorescent protein. Used as a control for autofluorescenceDepositorInsertScaffold (SH3pep-GS-LZX) protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH003
Plasmid#80397PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experimentsDepositorUseSynthetic BiologyTagsFused by GS linker to LZx domain and Venus has N-…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH015
Plasmid#80398PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blotsDepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH009
Plasmid#80399PurposeControl plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.DepositorUseSynthetic BiologyTagsC-terminal LZx domain and N-terminal 3xFLAG tag, …ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH016
Plasmid#80400PurposeNo anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).DepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVH004
Plasmid#80401PurposeContains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.DepositorUseSynthetic BiologyTagsC-terminal LZx domainExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF1-V5-His6
Plasmid#67411PurposeExpression of hArf1 with C-term V5 and His6 tag in E. coliDepositorInsertARF1 (ARF1 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF3-V5-His6
Plasmid#67412PurposeExpression of hArf3 with C-term V5 and His6 tag in E. coliDepositorInsertARF3 (ARF3 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF4-V5-His6
Plasmid#67413PurposeExpression of hArf4 with C-term V5 and His6 tag in E. coliDepositorInsertARF4 (ARF4 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF5-V5-His6
Plasmid#67414PurposeExpression of hArf5 with C-term V5 and His6 tag in E. coliDepositorInsertARF5 (ARF5 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailabilityAcademic Institutions and Nonprofits only
Showing: 6421 - 6440 of 27103 results