Addgene

Showing: 581 - 600 of 28122 results

Results per page:

Sort results by:

pET21-pelB-LaG-10

Plasmid

#172743

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-10

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-pA-Hia5

Plasmid

#174372

Purpose

SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein A - hia5

Use

Tags

6xHis and Protein A

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHis APEX2-eIF4A1

Plasmid

#129645

Purpose

Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

eukaryotic translation initiation factor 4A1 (EIF4A1 Human)

Use

Tags

6xHis and APEX2

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW003-PpsbA-mNeonGreen

Plasmid

#205019

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a-CA(A14C, E45C)

Plasmid

#79045

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C)

Use

Tags

Expression

Bacterial

Mutation

A14C and E45C mutations in HIV-1 CA protein

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AURKA_HUMAN_D0

Plasmid

#79739

Purpose

This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AURKA (AURKA Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSC5GGv1

Plasmid

#194645

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-18

Plasmid

#172763

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-18

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-42

Plasmid

#172758

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-42

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PthrC3_8

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-16-3xFLAG-LaG-2

Plasmid

#172765

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-3xFLAG-LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5238

Plasmid

#128711

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopG1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5052

Plasmid

#128729

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAM1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5951

Plasmid

#128719

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

hopR1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP3416

Plasmid

#128720

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopT1-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5534

Plasmid

#128723

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopX1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWJ1

Plasmid

#90484

Purpose

For gene disruption in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

pIM13 Origin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5372

Plasmid

#128733

Purpose

Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

Depositor

Alan Collmer

Article

Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5108

Plasmid

#128726

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAA1-2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge