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Plasmid#118572PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-pBAD
Plasmid#54667PurposeLocalization: Protein Expression Vector , Excitation: 584, Emission: 607DepositorTypeEmpty backboneUseTags6xHis and mRFP1ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcYbbN pET15b
Plasmid#60690PurposeRecombinant protein expression of YbbNDepositorInsertYbbN
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B YFP
Plasmid#108856PurposeEncodes for the fluorescent protein enhanced YFP (eYFP)DepositorInserteYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIB166
Plasmid#90189PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDUET-1-alpha-synuclein-mCerulean3-His6
Plasmid#110061PurposeFor bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidinesDepositorInsertalpha-synuclein (SNCA Human)
UseTags6 x His and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
UseTagsStrep-Tag II SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Cre
Plasmid#123128PurposeBacterial expression plasmid for wild-type Cre with an N-terminal His-tag.DepositorInsertCre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET PPL His6 LIC cloning vector (2J-T)
Plasmid#37182DepositorTypeEmpty backboneUseTagsHis6 and PPLExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pG1C
Plasmid#71740PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-PR/PRM-GFP
Plasmid#80859PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationPromoterLambda Pr and Lambda PrmAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZSm45 ndhII
Plasmid#48889PurposeLux-regulated expression of NDH-2 enzymeDepositorInsertndhII and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 EGFP-Sumo2 GG
Plasmid#13384DepositorInsertSumo2 GG (SUMO2 Human)
UseTagsEGFP and GSTExpressionBacterialMutationMature SUMO2GG: diglycine at amino acid positions…PromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAN3938
Plasmid#74697PurposeCircuit plasmid: 0xF6DepositorInsertCircuit 0xF6
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
6CC8 (MBD3)
Plasmid#119959PurposeBacterial expression for structure determinationDepositorInsertMBD3 (MBD3 Human)
UseTagsGSTtag, 6xHis and ThrombinExpressionBacterialMutationMBD domain (contains residues 1-71)PromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only