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Plasmid#178983PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)DepositorInsertTPR-CHAT
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE32-pr-mEosFP (mEosFP-V69T)
Plasmid#99213PurposeBacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).DepositorInsertpr-mEosFP
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T for primed c…PromoterT5Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a IHF
Plasmid#26641DepositorInsertIHF
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-HotPETase
Plasmid#218701PurposeEncodes codon optimized HotPETase enzyme for bacterial expressionDepositorInsertHotPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-DepoPETase
Plasmid#218702PurposeEncodes codon optimized DepoPETase enzyme for bacterial expressionDepositorInsertDepoPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
UseTags6xHis tag and HRV3C cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStrepIHFb
Plasmid#149385PurposeExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbetaDepositorInsertPseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
UseTagsHRV3C cleavage site and StrepII-tag withExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 FNBP3 WW domain #1
Plasmid#104233PurposeBacterial expression of WW domain #1 from FNBP3DepositorInsertFNBP3 WW domain #1 (PRPF40A Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS3
Plasmid#109287PurposeExpresses proteins in mycobacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNew promoter/cloning sitePromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
HERC2 (3KCI)
Plasmid#25344PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertherc2.3951.4321 (HERC2 Human)
UseTagsHisExpressionBacterialMutationcontains amino acid residues 3951-4321PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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SARS-CoV-2_N_FL
Plasmid#157867Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid proteinDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
UseTagsExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
NFAT5 luciferase
Plasmid#14110DepositorInsertNM_001113178.2 (NFAT5 Human)
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MEK1-DD
Plasmid#47576PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1DepositorInsertMEK1-DD (Map2k1 Mouse)
UseTags3xHA and GSTExpressionBacterialMutationSer218 to Asp, Ser221 to AspPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKD077
Plasmid#136473PurposeSSB-mTur2 IDL fusion expression plasmidDepositorInsertSSB-mTur2 (ssb E. coli)
UseTagsmTurquoise2ExpressionBacterialMutationmTurquoise2 inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterSP44sAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ETV2_pet28a
Plasmid#131665PurposeBacterial expression of ETS gene ETV2DepositorInsertETV2 (ETV2 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only