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pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Use

Tags

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor T19N (RHOA Human)

Use

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

Plasmid

#119946

Purpose

Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Renilla luciferase

Use

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Mutation

Promoter

TetCMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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SRpHi2

Plasmid

#102542

Purpose

Bacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-EGFP

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pRSETB

Plasmid

#48203

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Use

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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ALFY-FYVE 1G

Plasmid

#197045

Purpose

Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

Insert

ALFY (WDFY3 Human)

Use

Tags

6xHis-GST-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBL090J

Plasmid

#195045

Purpose

cCadC-CCB3.5 under the control of PBAD

Depositor

Tina Wang

Article

Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

Insert

cCadC-CCB3.5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

Plasmid

#191635

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadR)-DadR

Plasmid

#191636

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ZFYVE9C-c009

Plasmid

#53618

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

ZFYVE9C

Use

Tags

His-6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pJS102

Plasmid

#118281

Purpose

Expresses GFPmut2 with low cell-to-cell variation and lactose induction

Depositor

Zach Hensel

Article

Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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UBC32

Plasmid

#25213

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubc32.001.158 (UBE2K Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pTGD

Plasmid

#88894

Purpose

Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli

Depositor

Keith Tyo

Article

Love et al Methods Mol Biol. 2019;1927:37-45. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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p44K

Plasmid

#45800

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBADM11-HMGR

Plasmid

#108946

Purpose

heterologous expression of HMGR of S. solfataricus in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

HMG-CoA reductase (HMGR)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Use

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pET28-Rsn2

Plasmid

#61686

Purpose

bacterial expression of ranaspumin 2 as a His-tagged fusion protein

Depositor

Brian Smith

Article

Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

Insert

Ranaspumin-2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSR357.10

Plasmid

#125106

Purpose

Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGST-PSP-hTG

Plasmid

#85470

Purpose

bacterial expression of human tissue transglutaminase

Depositor

Jeffrey Keillor, Ray Truant

Article

Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

Insert

hTG2 (TGM2 Human)

Use

Tags

GST and PSP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-147

Plasmid

#118563

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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