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Showing: 4981 - 5000 of 28150 results

  4. pTP434

    Plasmid
    #104167
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    Use
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    3x Cysteines located at bps 493-495, 520-522, and…
    Promoter
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104167-plasmid-map-sequence-id-200061

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  5. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  8. pET32bplus_trxAChEwt

    Plasmid
    #83916
    Purpose
    Encodes an E. coli codon optimized version of wild type human acetylcholinesterase
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Short form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    codon optimised for E.coli expression
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83916-plasmid-map-sequence-id-174926

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  10. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Use
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37276-plasmid-map-sequence-id-46873

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  16. pKD072

    Plasmid
    #136472
    Purpose
    SSB-C-term-GFP fusion expression plasmid
    Depositor
    James Keck
    Article
    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60
    Insert
    SSB-C-term-GFP (ssb E. coli)
    Use
    Tags
    superfolder GFP
    Expression
    Bacterial
    Mutation
    superfolder GFP attached to C-terminus at Phe 178
    Promoter
    T7 promoter
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136472-plasmid-map-sequence-id-267962

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  17. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  19. pAV125

    Plasmid
    #63191
    Purpose
    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    63191-plasmid-map-sequence-id-104862

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Showing: 4981 - 5000 of 28150 results