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Plasmid#177660PurposeRecombinant protein synthesis of human HSPA1LDepositorInsertHSPA1L (HSPA1L Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b-17X-PTDH
Plasmid#166786PurposePhosphite dehydrogenase for NAD(P)H regenerationDepositorInsertPhosphite dehydroenase
UseTagsHis6ExpressionBacterialMutationA196R, T201S, A328T, E352N, and C356D- please see…PromoterT7Available sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB094e
Plasmid#79245PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4 (Y87A); PC araC
UseTagsExpressionBacterialMutationPromoterPBAD; PCAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR3-IR3-Bxb1-CadR-pZa
Plasmid#204686PurposeCadmium responsive genetic circuitDepositorInsertBxb1 (Bxb1p45 Synthetic)
UseSynthetic BiologyTagsCadRExpressionBacterialMutationPromoterInducible, HspR is its repressorAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1104
Plasmid#119248Purposeknockdown pqsC in P. aeruginosaDepositorInsertsgRNA pqsC (P. aeruginosa)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorInsertsUseTags6xHis and SUMOExpressionBacterialMutation2-116PromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRMs
Plasmid#157864Purposeexpress His tagged hnRNPA2 RRMsDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutation190stopPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ETS2_pet28a
Plasmid#131664PurposeBacterial expression of ETS gene ETS2DepositorInsertETS2 (ETS2 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-4T3-RhoA-Q63L
Plasmid#12961DepositorInsertRhoA constitutively active (RHOA Human)
UseTagsGSTExpressionBacterialMutationQ63L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJMD5
Plasmid#138652PurposephiBT1 integrating Streptomyces expression vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPermE* variant sequence from pDA1652Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-KIRIN1-GR
Plasmid#107417PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GRDepositorInsertKIRIN1-GR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRH2502
Plasmid#84379PurposeExpression of dcas9 D10A H840A from a TetR-regulated uvtetO promoterDepositorInsertdcas9
UseCRISPRTagsExpressionBacterialMutationAspartate at position 10 changed to Alanine, Hist…Promoteruv15tetOAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP2292
Plasmid#70707PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCK005.6
Plasmid#153025PurposedCas9, MCP-SoxS(R93A/S101A), J106 scRNADepositorInsertdCas9, MCP-SoxS(R93A/S101A), J106 scRNA
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2 Parkin RBR
Plasmid#31255DepositorInsertParkin (Prkn Rat)
UseTagsGSTExpressionBacterialMutationRBR domain, residues 217-465PromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-133
Plasmid#118551PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 141-465
Plasmid#45971DepositorInsertParkin 141-465 (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-Z-CGFP
Plasmid#52735PurposeL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assayDepositorInsertZ+CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only