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Plasmid#188327PurposePCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mCherry::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS011
Plasmid#188334PurposePCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mTagBFP2::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL004
Plasmid#180608PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertionDepositorInsertPlmCasX with DpbCasX R1 loop
UseCRISPRTags10xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK633
Plasmid#72397PurposeProduces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)DepositorInsertphosphoribosylaminoimidazole synthetase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK515
Plasmid#72385PurposeProduces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)DepositorInsertphosphoribosylglycinamide formyltransferase 2
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
Plasmid#85072PurposeGreen/red FRET biosensor for RhoA in the GTP locked constitutively active stateDepositorInsertRhoA Biosensor constitutively active (Q63L) (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR47S Cm
Plasmid#41892DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacbTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-PATagRFP
Plasmid#31945DepositorInsertPATagRFP
UseTagsHisExpressionBacterialMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoteraraBAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PIKFYVE_DEP
Plasmid#109789PurposeProtein expression and purification of PIKFYVE_DEPDepositorInsertPIKFYVE_DEP (PIKFYVE Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p47PX(aa2-132)
Plasmid#19007DepositorInsertp47phox (NCF1 Human)
UseTagsGSTExpressionBacterialMutationaa2-132 (PX domains)PromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEB1-wtGFP
Plasmid#103978PurposePlasmid encoding wtGFPDepositorInsertwtGFP
UseLow copyTagsExpressionBacterialMutationPromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRT7 NT Topo arabinose
Plasmid#73446PurposeUsed for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptidesDepositorTypeEmpty backboneUseTagsGST (N-terminal), 6xHis (C-terminal)ExpressionBacterialMutationPromoterpBadAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL1B
Plasmid#183386PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL1B (Il1b Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pColE1-ISceI
Plasmid#179578PurposeExpresses I-SceI from an arabinose promoterDepositorInsertI-SceI (I-SceI Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAT2.2
Plasmid#35621DepositorTypeEmpty backboneUseTags6xHIS, T7 tag, and TATExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDM2 (17-125)
Plasmid#62063PurposeExpression of human MDM2 (17-125) in e. coliDepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationcontains residues 17-125PromotertacAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
UseTags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…PromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry
Plasmid#145279PurposeBacterial Expression of 6xHis mCherry Fusion ProteinDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-STAT1
Plasmid#82722PurposeExpresses human STAT1DepositorInsertSTAT1 (STAT1 Human)
UseTags3xFLAG and GSTExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only