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Plasmid#86791Purposebacterial expression of mouse cyclin D2DepositorInsertcyclin D2 (Ccnd2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2-Rheb
Plasmid#15889DepositorInsertRheb (RHEB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24-1A(sfGFP)
Plasmid#126971PurposeLevel 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcD-SH2
Plasmid#46488DepositorInsertShcD (SHC4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 512-623PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3 I0500 LasI
Plasmid#107966PurposeArabinose-inducible 3OC12HSL sender plasmidDepositorInsertlasI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
UseTagsHis14 tagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB007
Plasmid#98995PurposeExpress FtsI tagged with tagRFP-t in E coliDepositorInsertFtsI
UseTagsTagRFP-TExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 884-1066-ECFP FRET
Plasmid#46179DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP, EYFP, and HisExpressionBacterialMutationaa 884-1066 (Vt, tail domain)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter
Plasmid#65007PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning siteDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoternoneAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30 SUMO-1 G97
Plasmid#13381DepositorInsertSUMO-1, G97 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationG97 mutant: lacks 4 amino acids from the C-termin…PromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human TBC1D7 (19-293)
Plasmid#132617Purposeexpresses human TBC1D7 (19-293) in E. coliDepositorInsertTBC1D7 (19-293)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Tn5
Plasmid#127917PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.DepositorInsertTn5
UseTagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7Available sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD87
Plasmid#119892PurposeThe pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpLPD03031_GFP+
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP11
Plasmid#215559PurposepKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 originDepositorInsertsCas9
gRNA targeting pBR322 origin of replication
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105 and tetAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTcI39-switch
Plasmid#195557PurposeTemperature state switch expressing low-temperature mWasabi and high-temperature mRFP1DepositorInsertsmRFP1
mWasabi
TcI39
UseTags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67TPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJS010
Plasmid#188333PurposePCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mKate2::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only