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pHH0103_RAB4B-2/2

Plasmid

#91554

Purpose

Protein expression and purification of human SH3 domain construct RAB4B-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RAB4B-2/2 (RAB4B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3KBP1-1/3

Plasmid

#91555

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-1/3 (SH3KBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHH0103_AMPH-1/1

Plasmid

#91556

Purpose

Protein expression and purification of human SH3 domain construct AMPH-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AMPH-1/1 (AMPH Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3RF2-2/3

Plasmid

#91557

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-2/3 (SH3RF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NOXO1-2/2

Plasmid

#91558

Purpose

Protein expression and purification of human SH3 domain construct NOXO1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXO1-2/2 (NOXO1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_BZRAP1-3/3

Plasmid

#91559

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-3/3 (TSPOAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS3/3

Plasmid

#91560

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS3/3 (ITSN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NCF4-1/1

Plasmid

#91561

Purpose

Protein expression and purification of human SH3 domain construct NCF4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF4-1/1 (NCF4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MPP5-1/1

Plasmid

#91562

Purpose

Protein expression and purification of human SH3 domain construct MPP5-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP5-1/1 (PALS1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_CACNB1-1/1

Plasmid

#91563

Purpose

Protein expression and purification of human SH3 domain construct CACNB1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB1-1/1 (CACNB1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAOJ15

Plasmid

#91567

Purpose

Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tetracycline promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAM_AraC

Plasmid

#91569

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Insert

AraC/PBAD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAM_Cam

Plasmid

#91570

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

Depositor

Harry Mobley

Article

Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-pNL29

Plasmid

#91696

Purpose

Expresses Mega GVs in E.Coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

Insert

Mega GV gene cluster (from pNL29) in pST39 backbone

Use

Tags

No tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-CohIII-CBM-HIS-LPETGG

Plasmid

#91697

Purpose

CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

CohIII-CBM-HIS-LPETGG

Use

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GGG-HIS-CBM-Xmod-DocIII

Plasmid

#91698

Purpose

CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-HIS-CBM-Xmod-DocIII

Use

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSupAR-MbPylRS(DiZPK)

Plasmid

#91705

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

Depositor

P. Chen

Article

Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

Inserts

pylS
pylT

Use

Tags

Expression

Bacterial

Mutation

T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

Promoter

Availability

Academic Institutions and Nonprofits only

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pMH94

Plasmid

#91720

Purpose

Integration-proficient vector for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin

Depositor

Graham Hatfull

Article

Lee et al Proc Natl Acad Sci U S A. 1991 Apr 15;88

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge