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t1-405

Plasmid

#166127

Purpose

For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

Depositor

Vesa Hytönen

Article

Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

Insert

Tln1Head (1-405) (TLN1 Human)

Use

Tags

His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

Expression

Bacterial

Mutation

residues 1-405 only

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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mNeptune-pBAD

Plasmid

#54714

Purpose

Localization: Protein Expression Vector , Excitation: 600, Emission: 650

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien, Robert Campbell

Article

Lin et al Chem Biol. 2009 Nov 25;16(11):1169-79. d

Type

Empty backbone

Use

Tags

6xHis and mNeptune

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMyBADC

Plasmid

#84688

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Bacterial expression vector

Tags

His6

Expression

Bacterial

Mutation

Promoter

Arabinose

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAraTMDN

Plasmid

#47515

Purpose

Contains A2B L980A TMCY mutant, expresses DN AraC (AraC*) for heterodimer competition assay

Depositor

Bryan Berger

Article

Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

Insert

Integrin alpha2B L980A TM-CYTO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-150

Plasmid

#118532

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-Rac1-Q61L

Plasmid

#12979

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pBad-PhoCl2f

Plasmid

#164036

Purpose

Improved PhoCl variant with faster dissociation rate

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl2f

Use

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Mutation

Promoter

araBad promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor Q63L (RHOA Human)

Use

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCDF-mTET1

Plasmid

#81052

Purpose

bacterial expression of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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p3187 HPV-16 E2

Plasmid

#10846

Depositor

Peter Howley

Article

Yasugi et al J Virol. 1997 Feb . 71(2):891-9.

Insert

HPV-16 E2 (E2 Human papillomavirus)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTD-NStrepHis

Plasmid

#45936

Purpose

use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Use

Tags

HIS-6-tag and Twin-Strep-tag

Expression

Bacterial

Mutation

Promoter

lacIq-Ptrc

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pACM4

Plasmid

#49797

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pBbE7a-RFP

Plasmid

#35313

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMal-Abl-PRM 5R

Plasmid

#112088

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-5R (ABL1 Human)

Use

Tags

His-6 and MBP

Expression

Bacterial

Mutation

construct contains only the PRM motif of Abl1

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM p21

Plasmid

#8443

Depositor

Frederic Mushinski

Article

Huppi et al Oncogene 1994 Oct;9(10):3017-20.

Insert

Mouse p21 (Cdkn1a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pHLUK

Plasmid

#64181

Purpose

harbours marker genes HIS3, LEU2, URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 Q331K

Plasmid

#27465

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Tags

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Synaptojanin 1-145

Plasmid

#22291

Depositor

Pietro De Camilli

Article

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Use

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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