-
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS4
Plasmid#60785PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS4
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pRSETB
Plasmid#109484Purposebacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
UseTagsN-terminal histidine tag in vectorExpressionBacterialMutationPromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY5
Plasmid#21764DepositorInsertECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCre-SacB-zeo
Plasmid#107706PurposeExpresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.DepositorInsertble (Zeo resistance)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp3_Unique
Plasmid#167258PurposeBacterial expression of Nsp3 Macrodomain 2 and Macrodomain 3 (residues 413-676)DepositorInsertNsp3 (residues 413-676) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorUseTagsHis tagExpressionBacterialMutationD181NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSETT4gap
Plasmid#153413PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotergapDH promoter from Eggerthella lentaAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30-RT1306
Plasmid#131521PurposeExpresses engineered RT-1306 with His-tagDepositorInsertRT-1306
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB202
Plasmid#101674PurposeOpto-T7RNAP(563)DepositorInsertOpto-T7RNAP(563)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK cyclin T1 (P#468)
Plasmid#14607DepositorInsertcyclin T1 (CCNT1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pDDRFP-A1
Plasmid#36289DepositorInsertDimerization dependent RFP A1
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJLG038
Plasmid#192981PurposeBHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoterDepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO3
Plasmid#52260PurposeBacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhdeA-cfp
Plasmid#127940PurposeCFP fluorescent reporter for HdeADepositorInsertPhdeA-cfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only