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Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_ATRX
Plasmid#221377PurposeExpresses ATRX in bacterial cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationchanged G736 to EPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEO100c
Plasmid#50550PurposeExpresses sfGFP under the PompC1157 promoterDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFTK020
Plasmid#171292PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPalcA syn_NoCrea (ANIA_08979) promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJS009
Plasmid#188332PurposePCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mKate2::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-UBE2S-UBD
Plasmid#66713PurposeExpresses the UBE2S-UBD fusion protein in E. coliDepositorUseTagsGST-3CExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
UseTagsFLAG purification tag and eGFPExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJS003
Plasmid#188326PurposePCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mCherry::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL4529 (pTnpA)
Plasmid#210869PurposeExpresses GstTnpA from pLac promoterDepositorInsertGstTnpA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB2Hopt
Plasmid#163830PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1BDepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRB035
Plasmid#181944PurposeaTc-inducible expression of mNeonGreenDepositorInsertmNeonGreen; tetR (AX04_RS23485 Synthetic)
UseTagsExpressionBacterialMutationPromotermNG-PLtetO-1; tetR-J23106Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only