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Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorInsertmEGFP-H1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
MS2-Snapf
Plasmid#65860PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tagDepositorInsertsMS2
Fast SNAP
UseTagsHA and NLSExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSt-KO
Plasmid#44566Purposefor generation of knockout of mycobacterial genesDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
FEV_pet28a
Plasmid#131668PurposeBacterial expression of ETS gene FEVDepositorInsertFEV (FEV Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKCiR5
Plasmid#149462PurposeBroad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP3664
Plasmid#222350PurposeExpression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12068
Plasmid#222356PurposeTn7 integrative vector with PabstBR promoter expression cassette and kanR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12049
Plasmid#222355PurposeTn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPabstBRAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12042
Plasmid#222354PurposeTn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204798PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204816PurposeBacterial expresison of Enterovirus A71 3C proteaseDepositorInsertEV A71 3C protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAJT248
Plasmid#202289PurposeRPU integration plasmid (LPattB2)DepositorInsertJ23101-RiboJ00-B0034-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETNb-nALFA
Plasmid#159988PurposeFor the protein expression with ALFA tag at N-terminus in E. coliDepositorTypeEmpty backboneUseTagsMVKYI-8histidine-ALFA tag-HRV3C protease cleavage…ExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-ecDHFR (WT)
Plasmid#73188Purposebacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)DepositorInsertfolA dihydrofolate reductase (folA E. coli)
UseTagsHis 6XExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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ETS1_pet28a
Plasmid#131663PurposeBacterial expression of ETS gene ETS1DepositorInsertETS1 (ETS1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ20
Plasmid#65027PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits