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Showing: 4461 - 4480 of 28150 results

  2. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid
    #114069
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114069-plasmid-map-sequence-id-220520

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  11. pcDNA3.1.GĪ±13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of GĪ±13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of GĪ±13. Addition of the HA epitope at N term of GĪ±13.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    GĪ±13-LgB106 (GNA13 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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  14. pHis17_ParM _2

    Plasmid
    #78202
    Purpose
    Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
    Depositor
    Martin Webb
    Article
    Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.
    Insert
    parM (K33A D63C T174A T175N D224C C287A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    K33A D63C T174A T175N D224C C287A
    Promoter
    unknown
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    78202-plasmid-map-sequence-id-146031

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  16. pET28a-His-MinD_MinE

    Plasmid
    #133621
    Purpose
    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
    Depositor
    Petra Schwille
    Article
    Loose et al Science. 2008 May 9;320(5877):789-92. do
    Insert
    His-MinD_MinE (minD, minE E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133621-plasmid-map-sequence-id-271463

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  19. pRB117.2

    Plasmid
    #181949
    Purpose
    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181949-plasmid-map-sequence-id-356570

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  20. pETM-41-HsNot6_N

    Plasmid
    #147011
    Purpose
    Bacterial Expression of HsNot6
    Depositor
    Elisa Izaurralde
    Insert
    HsNot6 (CNOT6 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147011-plasmid-map-sequence-id-446215

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Showing: 4461 - 4480 of 28150 results