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Plasmid#91720PurposeIntegration-proficient vector for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-GuerinDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only
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pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-eLACCO1.1
Plasmid#167944PurposeFluorescent biosensor for extracellular L-lactateDepositorInserteLACCO1.1
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFD_L
Plasmid#198276PurposeFor loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_L
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBroad-T7RNAP-chlorR
Plasmid#199992PurposeT7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorRDepositorInsertT7RNAP Circuit
UseTagsExpressionBacterialMutationcopFnullPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (23-111)
Plasmid#62064PurposeExpression of human MDM4 (residues 23-111) in e. coliDepositorInsertMDM4 (MDM4 Human)
UseTagsGSTExpressionBacterialMutationcontains residues 23-111PromotertacAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3113 GST-p53
Plasmid#10852DepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
GLS2A-c007
Plasmid#53621Purposebacterial expression plasmidDepositorInsertGLS2A
UseTagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mouse ptc probe M2-3
Plasmid#58701Purposemakes mouse ptc probe for in situ hybridizationDepositorInsertptc (Ptch1 Mouse)
UseIn situ probeTagsExpressionBacterialMutationonly EcoRI fragment (5' end)PromoterT7Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah1
Plasmid#122568PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP-T
Plasmid#103998PurposePlasmid encoding TagRFP-TDepositorInsertTagRFP-T
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)GFP-9xGGS-Cre-6xHis
Plasmid#62372PurposeExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cellsDepositorInsert(-30)GFP-9xGGS-Cre-6xHis
UseTags(-30)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53SS(1-393)
Plasmid#200311PurposeBacterial expression of human superstable p53 as His6GB1tev fusionDepositorInsertp53 (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4
Plasmid#21156DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
A-BRDA4-3
Plasmid#166513PurposescFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 333-460 of 1362, Bromodomain 2DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR78
Plasmid#90210PurposeExpresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyADepositorInsertsCcaS
Cph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM427
Plasmid#210788PurposeL5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transferDepositorInsertDefective hygromycin resistant gene
UseTagsExpressionBacterialMutationChanged hygromycin resistant gene W190 codon (TGG…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_sensor_GFP
Plasmid#139463PurposeT7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporterDepositorInsertSNIPR_GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB070.iodo.5
Plasmid#99397PurposeExpression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanineDepositorInsertPN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
UseTagsExpressionBacterialMutationPromoterPN25, Ptet, and PproKAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only