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Plasmid#199779PurposeUsed for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)DepositorInsert4X Ubiquitin in Tandem
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHuji
Plasmid#61555PurposeRed fluorescent protein based pH indicator in bacterial expression vectorDepositorInsertpHuji
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT13
Plasmid#200993PurposepLv1-pUC18R6KT-miniTn7-Tc, TcR, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.G
Plasmid#200999PurposepLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT13.G
Plasmid#201001PurposepLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT14
Plasmid#201015PurposepLv1-pUC18R6KT-miniTn7-aad9, SpR, StR, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT10
Plasmid#200989PurposepL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT12
Plasmid#200992PurposepLv1-pUC18R6KT-miniTn7-Km, KmR, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP21 (3MTN)
Plasmid#28112PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP21 (USP21 Human)
UseTagsHis tag with Thrombin cleavageExpressionBacterialMutationcatalytic domain only (amino acid residues 209-56…PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
csr-1_son-1_mTagBFP2
Plasmid#191746PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mTagBFP2ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)
Plasmid#181789PurposeExpresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTniQ, ShCas12k-ShTnsC
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA620
Plasmid#186240PurposeLbuCas13a Negative Control (RFP targeting)DepositorInsertpTet-eLbuCas13a + crRNA negative control
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCascade
Plasmid#140622PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNADepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-X0
Plasmid#115565PurposeControl vector expressing GFP alone without any consensus repeatsDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationPromoterADH1Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaD (pESC106)
Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
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YPet-pBAD
Plasmid#54860PurposeLocalization: Protein Expression Vector , Excitation: 513, Emission: 527DepositorTypeEmpty backboneUseTags6xHis and YpetExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmr101A-hVPS26
Plasmid#17636DepositorInsertVPS26 (VPS26A Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xHA
Plasmid#129567PurposeEmpty backbone for HA tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only