-
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
UseTags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEE065
Plasmid#176822PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert704
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET14b – lambda exonuclease
Plasmid#104531Purposebacterial expression of lambda exonucleaseDepositorInsertλ exonuclease
UseTagsHis6 followed by a thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOmpF
Plasmid#42606DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT2/BH
Plasmid#26556DepositorInsertpT2/BH
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO3
Plasmid#53143PurposeE.coli expression of untagged mature human SUMO3 (amino acids 1-93)DepositorInsertSUMO3 (SUMO2 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-93)PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-tsapphire
Plasmid#78464PurposeBroad range bacterial fluorescence labelingDepositorInsertT-Sapphire
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMyrPalm_mEGFP_IRES_puro2b
Plasmid#21038DepositorInsertpMyrPalm
UseTagsmEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-E488Q-V5
Plasmid#139685PurposeExpresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assaysDepositorInsertHrp48, ADAR (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-4F2-mCherry
Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP4F2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT004-LaG30VB
Plasmid#163384Purpose2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN systemDepositorInsertLaG30-VEEGFRtm-BFP (LaG30VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHN1_lacUV5-mNG-PDT
Plasmid#165583PurposeExpresses mNeonGreen and C-terminally fused PDTDepositorInsertmNeonGreen-PDT
UseTagsProtein degradation tagExpressionBacterialMutationPromoterPtrcAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII
Plasmid#105839Purposeexpresses E. coli HPII catalase with N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
UseTags6x his tagExpressionBacterialMutationPromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT03-Apr
Plasmid#154756PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, AprarDepositorInsertFLP recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
p405TEF1
Plasmid#15968DepositorInsertTEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationTEF1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-3-6AOptParkin
Plasmid#165107Purposebacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin geneDepositorInsertParkin isoform 1 (PRKN Human)
UseTagsGST-6-alanineExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-Itch/2793
Plasmid#11434DepositorInsertItch (Itch Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_Smc4-mAID-HaloTAG
Plasmid#217658PurposeRepair template to endogenously tag SMC4 with mAID-Halo TagDepositorInsertSMC4-mAID-HaloTag (SMC4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF1 (WT)
Plasmid#175413PurposeEncodes His-MBP-TEV-WT eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
UseTagsHis-MBP-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only