Skip to main content
Addgene
Showing: 21 - 40 of 126 results

  1. pSaPIPLC His

    Plasmid
    #173810
    Purpose
    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87
    Insert
    Staphylococcus aureus plc
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173810-plasmid-map-sequence-id-341162

    Enlarge

  5. pET15b-Fab1 (S. aureus)

    Plasmid
    #61691
    Purpose
    Inducible expression of the FabI enzyme from S. aureus
    Depositor
    Michael Johnson
    Insert
    FabI
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pET15b-ACP (from S. aureus)

    Plasmid
    #63686
    Purpose
    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus
    Depositor
    Michael Johnson
    Insert
    ACP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

    Enlarge

  19. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

    Enlarge

Showing: 21 - 40 of 126 results