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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TEF

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMUT2-VtrA-mCherry

Plasmid

#192862

Purpose

pMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pPIPL12-FnCas12a

Plasmid

#199563

Purpose

Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

Fncas12a

Use

CRISPR and Synthetic Biology; Tetracycline induci…

Tags

Expression

Bacterial

Mutation

Promoter

COtetR-PIPL12

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pthl_fU

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_FLAG-pvc2

Plasmid

#198285

Purpose

Contains an external epitope tag on the sheath protein; for immunofluorescence-based binding assays

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_FLAG-pvc2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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PARD3_PDZ_3

Plasmid

#103959

Purpose

Protein expression and purification of PAR3 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PARD3 (PARD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-bphC

Plasmid

#128137

Purpose

The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

bphC

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGex-4T-2_GABARAP

Plasmid

#73948

Purpose

For recombinant expression of human GABARAP in E.coli

Depositor

Dieter Willbold

Article

Stangler et al J Biomol NMR. 2001 Oct;21(2):183-4.

Insert

GABARAP (GABARAP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pGI3EM20C

Plasmid

#135487

Purpose

Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr H2B-tdTomato

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[himar1C9]

Plasmid

#213906

Purpose

Mariner transposon with ATc-inducible himar1C9 transposase

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and ATc-inducible himar1C9 transposase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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JMJD3

Plasmid

#39069

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JMJD3 (KDM6B Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pTD103aiiA

Plasmid

#48884

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Danino et al Nature. 2010 Jan 21;463(7279):326-30. do

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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PARP3A

Plasmid

#42380

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PARP3A (PARP3 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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GST-FUS-WT

Plasmid

#44978

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pGST-DHFR Mm

Plasmid

#83073

Purpose

Expresses the full length DHFR Mm in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

2x DHFR (Dhfr Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCAP01

Plasmid

#59981

Purpose

The function of this material is to facilitate the capture and expression of bacterial gene clusters.

Depositor

Bradley Moore

Article

Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Type

Empty backbone

Use

Synthetic Biology; Transformation-associated reco…

Tags

no

Expression

Bacterial and Yeast

Mutation

Promoter

no

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pST-2K

Plasmid

#44564

Purpose

for ectopic constitutive expression of two mycobacterial proteins in one vector

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Human HUWE1 (3993-4374)

Plasmid

#110754

Purpose

Bacterial Expression of HUWE1 HECT domain

Depositor

David Komander

Article

Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

Insert

HUWE1 (HUWE1 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Article

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Use

Tags

6xHis-tag and GFP11

Expression

Bacterial

Mutation

Promoter

pARA

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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