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Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
UseTagsExpressionBacterialMutationGvpA T6A-I48VPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015
Plasmid#202025PurposeExpresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coliDepositorInsertBacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
UseTagsExpressionBacterialMutationGvpB S9G-R31L-R85LPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA
Plasmid#176745PurposeExpresses Glt-1 fused with BioID2 at the C-terminus in astrocytesDepositorInsertGlt1-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pST-K
Plasmid#44560Purposefor ectopic constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
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(SUMO)5
Plasmid#126949PurposeBacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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dFP-mini
Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotertac promoter from pKK233-2Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_DARPinOFF7
Plasmid#182396PurposeBacterial expression of DARPinOFF7DepositorInsertDARPinOFF7
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)
Plasmid#130640PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on TniQExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCoofy48
Plasmid#55186PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…ExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2B
Plasmid#15781DepositorInsertUbiquitin conjugating enzyme UbE2B (UBE2B Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only