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Plasmid#89241PurposeResurfaced Cell-penetrating GFP nanobodyDepositorInsertpcNB1 (GFP)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
UseTagsStrepIIExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCE35
Plasmid#174409PurposeC. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXW117Ptet2-gfp
Plasmid#160818PurposeaTc-responsive single-layered circuitDepositorInsertPJ23117-30tetR-t-Ptet2-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23117, Ptet2Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET-Neq2X
Plasmid#182365PurposeExpresses Neq2X DNA polymeraseDepositorInsertNeq2X
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-DogCatcher
Plasmid#171929PurposeExpress Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.DepositorInsertCys-DogCatcher
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCis mCherry-SS Himar1 A7
Plasmid#84687PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-630)
Plasmid#171086PurposeCo-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorUseTagsHisExpressionBacterialMutationDeleted N-term 26aa and C-term 142aa for mutant e…PromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(400-772)
Plasmid#171087PurposeCo-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorUseTagsHisExpressionBacterialMutationDeleted N-term 26+399 amino acids for mutant expr…PromoterT5 promoter and T7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIE
Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTFIIB
Plasmid#171084PurposeExpresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIBDepositorInsertTF2B (GTF2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-772)
Plasmid#171085PurposeCo-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorUseTagsHisExpressionBacterialMutationDeleted N-term 26 amino acids for expression purp…PromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-STING(pLxIS)
Plasmid#221333PurposeOverexpression of STING CTT in bacteriaDepositorInsertSTING CTT (Sting1 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only