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Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(NC)-SH2
Plasmid#46471DepositorInsertPLCg1(NC) (PLCG1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 542-759PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH385-GST-TEV-SUP35
Plasmid#29744DepositorInsertSUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
UseTagsGSTExpressionBacterialMutationwild typePromoterAvailable sinceAug. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFnCas12a_NT
Plasmid#169838PurposeExpresses FnCas12a and guide RNA in BacteriaDepositorInsertFnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterpXynAAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO1
Plasmid#24316DepositorInserthuman AGO1 (AGO1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
p2B-58
Plasmid#177928PurposeExpresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)DepositorArticleInsertEGFP
UseTagsExpressionBacterial and YeastMutationPromoterTET4 (contains four rtTA binding sites)Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIV pm
Plasmid#218101PurposePhagemid that expresses LacI, and pIV under the Lac promoterDepositorInsertpIV
UseTagsExpressionBacterialMutationPromoterLac promoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPro24-gfp
Plasmid#18880DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-POLR2A-CTD-mCherry
Plasmid#185011PurposeBacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion ProteinDepositorInsertPOLR2A (Polr2a Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD227
Plasmid#90954PurposeExpresses S. baltica TtrS under the Ptac promoterDepositorInsertTetrathionate sensor kinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
katushka-FMDV-P2A-pTRIP
Plasmid#163522PurposeUsed to construct lentivirus to express katushka in mammalian cells with option for a second insertDepositorInsertstopless Katushka-P2A
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-TRP1
Plasmid#11007DepositorInsertTRP1 (TRP1 Budding Yeast)
UseTagsV5 and his6ExpressionBacterial and YeastMutationORF-TRP1 without stop codonPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pComb3XLambda
Plasmid#63892PurposeBackbone pComb3X containing PCR template for human antibody lambda light chain constant regionDepositorInserthuman antibody lambda light chain (IGLL5 Human)
UsePhage displayTagsExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-Caspase-4_enzymatic-domain
Plasmid#183381PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange
Plasmid#31886DepositorInsertPSmOrange
UseTagsExpressionBacterialMutationS21T, Q63L, F100Y, L125M, K166R, P192S (mutations…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQmod2C-GG
Plasmid#191347PurposeClostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_AQUAR
Plasmid#204239PurposeBacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_AQUAR
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD69m1-TetW
Plasmid#191246PurposeE. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lentaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only